Measurement of Type B natriuretic peptide in heparin and K2 EDTA plasmaClinical Biochemistry，2010:in press
Background
Ethylene diamine tetraacetic acid (EDTA) plasma is the only suitable specimen recommended by the manufacturers to be used in the determination of BNP. It appears crucial to evaluate if more conventional heparin plasma samples could be reliably used for BNP determination. Aim of this study was to evaluate the use of heparin plasma sample for BNP determination.
Methods
Venous blood from 42 consecutive patients admitted at the division of cardiology was collected in two test tubes, with K2-EDTA (Group 1) and lithium heparin with gel separator (Group 2) and analysed within 20 min of blood collection.
Results
Statistical analysis showed a significant difference between Group 1 and Group 2 (p < 0.0001). Sample collected in K2-EDTA showed a significant underestimation when compared to lithium–heparin.
Conclusions
Our data showed that BNP could not be dosed on different collection tubes without altering the results. In our experimental conditions, interestingly we found that BNP levels are significantly lower if measured in EDTA plasma.
譯文：
在肝素抗凝和EDTA-K2抗凝血漿中測(cè)定B型利鈉肽（BNP）。
背景：在測(cè)定BNP中，生產(chǎn)廠家往往推薦EDTA抗凝血漿最為唯一適合的測(cè)定標(biāo)本。更加便捷分離血漿和血細(xì)胞的肝素抗凝血是否可以用于測(cè)定BNP是一個(gè)值得研究的問(wèn)題。我們研究的目的就是評(píng)價(jià)使用肝素抗凝血漿是否可以測(cè)定BNP的含量。
方法：收集了心臟病室的42名被連續(xù)觀察的病人的血漿，分別采用EDTA-K2抗凝（第一組）和帶有分離膠的肝素抗凝血漿（第二組）。在血漿收集的20分鐘內(nèi)測(cè)定。
結(jié)果：統(tǒng)計(jì)結(jié)果顯示兩組之間存在明顯差異（P<0.0001）。EDTA-K2抗凝標(biāo)本的結(jié)果明顯低于肝素抗凝的結(jié)果。
結(jié)論：數(shù)據(jù)表明利鈉肽B在不同的抗凝管收集后，測(cè)定結(jié)果會(huì)出現(xiàn)差異。在我們的試驗(yàn)結(jié)果里，如果采用EDTA-K2血漿的話，BNP的測(cè)定結(jié)果明顯偏低。
摘自《DXY》 |
